Publications

2024年

  1. 由里本博也、阪井康能第12章　葉圏C1細菌－植物間相互作用とバイオスティミュラント機能の開発バイオスティミュラントの開発動向と展望,pp.117-126, シーエムシー出版 (東京), (2024).

  2. 由里本博也、井上紘一、阪井康能C1酵母の濃度応答性メタノール誘導とペキソファジー制御におけるシグナル伝達化学と生物,62(9), 447-454 (2024).

  3. 由里本博也、阪井康能循環型C1バイオエコノミーで活用するメチロトロフの代謝生理機能環境バイオテクノロジー学会誌,24(1), 53-58 (2024).

  4. Kosuke Shiraishi, Yumi Arima, Motoharu Nakamura, Takumi Nakatsuji, Masahide Oku and Yasuyoshi Sakai.A novel fluorescence-activated cell sorting (FACS)-based screening identified ATG14, the gene required for pexophagy in the methylotrophic yeastFEMS Yeast Res., doi: 10.1093/femsyr/foae022.

  5. 阪井康能, 奥　公秀概論―ミクロオートファジーとは何か：研究の変遷と酵母で明らかになる分子機構実験医学,42(13), 2054-2062 (2024). doi: 10.18958/7529-00002-0001675-00.

2023年

  1. Yasuyoshi Sakai and Masahide Oku.ATG and ESCRT control multiple modes of microautophagyFEBS Lett.,doi:10.1002/1873-3468.14760

  2. Akio Tani, Sachiko Masuda, Yoshiko Fujitani, Toshiki Iga, Yuuki Haruna, Shiho Kikuchi, Wang Shuaile, Haoxin Lv, Shiori Katayama, Hiroya Yurimoto, Yasuyoshi Sakai, and Junichi Kato.Metabolism-linked methylotaxis sensors responsible for plant colonization in Methylobacterium aquaticum strain 22AFront. Microbiol.,14, 1258452 (2023). doi: 10.3389/fmicb.2023.1258452.

  3. Yasuyoshi Sakai, Hiroya Yurimoto, and Seigo Shima.Methane monooxygenases; physiology, biochemistry and structureCatal. Sci. Technol.,13, 6342 – 6354 (2023). doi: 10.1039/D3CY00737E

  4. Taiki Makizuka, Keisei Sowa, Shiori Katayama, Yuki, Kitazumi, Hiroya Yurimoto, Yasuyoshi Sakai, and Osamu Shirai.An enhanced direct electron transfer-type NAD+/NADH regenerating system using the diaphorase subunit of formate dehydrogenase 1Electrochim. Acta,465, 142954 (2023). doi: 10.1016/j.electacta.2023.142954.

  5. 阪井康能、由里本博也葉面における微生物 −宿主植物相互作用とバイオスティミュラント機能の開発−日本農薬学会誌,48(2), 132-136 (2023).

  6. 由里本博也C1バイオエコノミーで活用するメタノール酵母の遺伝子発現制御機構アグリバイオ,7(7), 621-623 (2023).

  7. 由里本博也、阪井康能メタノールからのバイオものづくり生物工学,101(5), 226-229 (2023). doi: 10.34565/seibutsukogaku.101.5_226

  8. 由里本博也、阪井康能メタン酸化菌とC1バイオエコノミーメタンと二酸化炭素　～その触媒的化学変換技術の現状と展望～,pp.59-66, シーエムシー･リサーチ (東京), (2023).

  9. Hiroya Yurimoto and Yasuyoshi Sakai.Interaction between C1-microorganisms and plants: contribution to the global carbon cycle and microbial survival strategies in the phyllosphereBiosci. Biotechnol. Biochem.,87: 1-6 (2023). doi: 10.1093/bbb/zbac176.

2022年

  1. Koichi Inoue, Shin Ohsawa, Shinji Ito, Hiroya Yurimoto, and Yasuyoshi Sakai.Phosphoregulation of the transcription factor Mxr1 plays a crucial role in the concentration-regulated methanol induction in Komagataella phaffiiMol. Microbiol.,118: 683-697 (2022). doi: 10.1111/mmi.14994.

  2. 由里本博也酵母細胞による異種タンパク質生産バイオプロセスを用いた有用性物質生産技術 ～動物・植物細胞や微生物の培養・分離精製技術～,pp.488-495, 技術情報協会 (東京), (2022).

  3. Patrick Otieno Juma, Yoshiko Fujitani, Ola Alessa, Tokitaka Oyama, Hiroya Yurimoto, Yasuyoshi Sakai, and Akio Tani.Siderophore for lanthanide and iron uptake for methylotrophy and plant growth promotion in Methylobacterium aquaticum strain 22AFront. Microbiol.,13: 921635 (2022). doi: 10.3389/fmicb.2022.921635.

  4. Shin Ohsawa, Masahide Oku, Hiroya Yurimoto and Yasuyoshi Sakai.Regulation of Peroxisome Homeostasis by Post-Translational Modification in the Methylotrophic Yeast Komagataella phaffiiFront. Cell Dev. Biol.,10: 887806 (2022). doi: org/10.3389/fcell.2022.887806.

  5. Kosuke Shiraishi and Yasuyoshi Sakai.Autophagy as a Survival Strategy for Eukaryotic Microbes Living in the PhyllosphereFront. Plant Sci.,13: 867486 (2022). doi: 10.3389/fpls.2022.867486.

  6. 白石晃將、阪井康能オートファジーからひも解く植物葉面微生物の生存戦略 植物のライフサイクルに伴う棲息環境の変化へ適応するために化学と生物,60(1), 2-4 (2022).

  7. 由里本博也菌は死んでも役に立つ　死菌体の出穂後１回散布で酒米の収量16％アップ現代農業,101(1), 78-79 (2022).

2021年

  1. 髙木　忍、由里本博也、阪井康能多様な炭素源を利用するメタノール資化性酵母の遺伝子発現系生物工学,99(10), 516-520 (2021). doi: 10.34565/seibutsukogaku.99.10_516

  2. 由里本博也、阪井康能メタノール細菌・細胞壁成分の出穂後葉面散布による酒米増収バイオサイエンスとインダストリー,79(4), 298-299 (2021).

  3. Pengli Ma, Shigeo Takashima, Chikako Fujita, Saya Yamada, Yusuke Oshima, Hao-Liang Cai, Hiroya Yurimoto, Yasuyoshi Sakai, Takashi Hayakawa, Masaya Shimada, Xia Ning, Baoyao Wei, and Tomoyuki Nakagawa.Fatty acid composition of the methylotrophic yeast Komagataella phaffii grown under low- and high-methanol conditionsYeast, 38: 541-548 (2021). doi: 10.1002/yea.3655.

  4. Viviane Carnier Casaroli, Izumi Orita, Shiori Katayama, Hiroya Yurimoto, Yasuyoshi Sakai, and Toshiaki Fukui.Methylotrophic bacterium-based molecular sensor for the detection of low concentrations of methanolJ. Biosci. Bioeng., 132: 247-252 (2021). doi: 10.1016/j.jbiosc.2021.05.002.

  5. Yuki Oku, Masahiro Kariya, Takaaki Fujimura, Jun Hoseki, and Yasuyoshi SakaiHomeostasis of the ER redox state subsequent to proteasome inhibitionSci. Rep., 11: 8655 (2021). doi: 10.1038/s41598-021-87944-y.

  6. Hiroya Yurimoto, Kosuke Shiraishi, and Yasuyoshi Sakai.Physiology of methylotrophs living in the phyllosphereMicroorganisms, 9: 809 (2021).doi: 10.3390/microorganisms9040809.

  7. Shin Ohsawa, Koichi Inoue, Takahiro Isoda, Masahide Oku, Hiroya Yurimoto, and Yasuyoshi SakaiThe methanol sensor Wsc1 and MAP kinase suppress degradation of methanol-induced peroxisomes in methylotrophic yeastJ. Cell Sci., 134: jcs.254714 (2021). doi: 10.1242/jcs.254714.

  8. Yurimoto H, Iguchi H, Di Thien DT, Tani A, Okumoto Y, Ota A, Yamauchi T, Akashi T, Sakai Y.Methanol bioeconomy: promotion of rice crop yield in paddy fields with microbial cells prepared from natural gas-derived C1 compound.Microb. Biotechnol., 14: 1385-1396 (2021). doi: 10.1111/1751-7915.13725.

2020年

  1. 由里本博也葉面メタノール細菌による作物増収技術の開発アグリバイオ,4(12), 1102-1104 (2020).

  2. 阪井康能酵母細胞センサー：メタノールの1細胞センシング技術 -生物学・資源循環科学・酵素工学への展開-化学と生物,58(7), 416-423 (2020).

  3. Abe Y, Honsho M, Kawaguchi R, Matsuzaki T, Ichiki Y, Fujitani M, Fujiwara K, Hirokane M, Oku M, Sakai Y, Yamashita T, Fujiki Y.A peroxisome deficiency-induced reductive cytosol state up-regulates the brain-derived neurotrophic factor pathway.J. Biol. Chem., 295: 5321-5334 (2020). doi: 10.1074/jbc.RA119.011989.

  4. 由里本博也、阪井康能合成生物学によるスーパーメタン酸化生体触媒の創製日本エネルギー学会機関誌 えねるみくす,99(2), 141-146 (2020).

  5. 奥　公秀、阪井康能ミクロオートファジー研究の課題医学のあゆみ,272(9), 856-860 (2020).

  6. 阪井康能メタノール資化性葉面共生菌による”C1炭素固定”と農学への展開農業および園芸,95(1), 27-32 (2020).

  7. Tomoyuki Takeya, Miyabi Yamakita, Daisuke Hayashi, Kento Fujisawa, Yasuyoshi Sakai, and Hiroya Yurimoto.Methanol production by reversed methylotrophy constructed in Escherichia coliBiosci. Biotechnol. Biochem., 84: 1062-1068 (2020). doi: 10.1080/09168451.2020.1715202.

2019年

  1. Masahide Oku and Yasuyoshi Sakai.Peroxisome Degradation and Its Molecular MachineryPeroxisomes: Biogenesis, Function, and Role in Human DiseasePart I, Chapter 3(2019).

  2. 阪井康能ようやく明らかになりつつあるミクロオートファジーの多様な分子機構生化学,91(5), 634-642 (2019).

  3. 由里本博也、阪井康能メタノールバイオエコノミー：C1 微生物代謝生理の分子基盤を活用した資源循環型社会の構築酵素工学ニュース,82, 26-31 (2019).

  4. Shinobu Takagi, Noriko Tsutsumi, Yuji Terui, XiangYu Kong, Hiroya Yurimoto, and Yasuyoshi Sakai.Engineering the expression system for Komagataella phaffii (Pichia pastoris): an attempt to develop a methanol-free expression systemFEMS Yeast Res., 19: foz059 (2019).doi: 10.1093/femsyr/foz059.

  5. Hiroya Yurimoto and Yasuyoshi Sakai.Methylotrophic yeasts: Current understanding of their C1-metabolism and its regulation by sensing methanol for survival on plant leavesCurr. Issues Mol. Biol., 33: 197-210 (2019).doi: 10.21775/cimb.033.197.

  6. Hiroyuki Iguchi, Ryohei Umeda, Hiroki Taga, Tokitaka Oyama, Hiroya Yurimoto, and Yasuyoshi Sakai.Community composition and methane oxidation activity of methanotrophs associated with duckweeds in a fresh water lake.J. Biosci. Bioeng., 128: 450-455  (2019). doi.org/10.1016/j.jbiosc.2019.04.009.

  7. Pang Y, Yamamoto H, Sakamoto H, Oku M, Mutungi JK, Sahani MH, Kurikawa Y, Kita K, Noda NN, Sakai Y, Jia H, Mizushima N.Evolution from covalent conjugation to non-covalent interaction in the ubiquitin-like ATG12 systemNat Struct Mol Biol., 26: 289-296 (2019). doi: 10.1038/s41594-019-0204-3.

  8. Fukuoka H, Kawase T, Oku M, Yurimoto H, Sakai Y, Hayakawa T, Nakagawa T.Peroxisomal Fba2p and Tal2p complementally function in the rearrangement pathway for xylulose 5-phosphate in the methylotrophic yeast Pichia pastorisJ Biosci Bioeng.,128: 33-38 (2019) doi.org/10.1016/j.jbiosc.2019.01.008.

  9. Yusuke Yoshida, Hiroyuki Iguchi, Yasuyoshi Sakai & Hiroya Yurimoto.Pantothenate auxotrophy of Methylobacterium spp. isolated from living plantsBiosci. Biotechnol. Biochem., 83: 569-577 (2019). doi.org/10.1080/09168451.2018.1549935.
    [農芸化学会 BBB論文賞]

2018年

  1. 奥　公秀オートファジーと脂肪滴中性脂質代謝Bio Clinica,33(7), 47-50 (2018).

  2. Shin Ohsawa, Susumu Nishida, Masahide Oku, Yasuyoshi Sakai, Hiroya Yurimoto.Ethanol represses the expression of methanol-inducible genes via acetyl-CoA synthesis in the yeast Komagataella phaffii.Sci. Rep.,8: 18051 (2018). doi: 10.1038/s41598-018-36732-2.

  3. Tomoyuki Takeya, Hiroya Yurimoto, Yasuyoshi Sakai.A Pichia pastoris single-cell biosensor for detection of enzymatically produced methanolAppl. Microbiol. Biotechnol., 102, 7017-7027 (2018). doi.org/10.1007/s00253-018-9144-9.

  4. Iguchi H, Yoshida Y, Fujisawa K, Taga H, Yurimoto H, Oyama T, Sakai Y.KaiC family proteins integratively control temperature-dependent UV resistance in Methylobacterium extorquens AM1Environ. Microbiol. Rep., 10, 634-643 (2018). doi.org/10.1111/1758-2229.12662.

  5. Masahide Oku Yasuyoshi Sakai.Three Distinct Types of Microautophagy Based on Membrane Dynamics and Molecular Machineries.BioEssays, 40, 180008 (2018). doi: 10.1002/bies.201800008.[Cover Image]

  6. 由里本博也、大澤　晋、阪井康能異種タンパク質生産に有用なC1酵母のメタノールセンシング機構バイオサイエンスとインダストリー,76(1), 30-34 (2018).

  7. Kosuke Shiraishi, Takahiro Hioki, Akari Habata, Hiroya Yurimoto, Yasuyoshi Sakai.Yeast Hog1 proteins are sequestered in stress granules during high-temperature stress.J. Cell Sci., 131, jcs209114 (2018). doi: 10.1242/jcs.209114.

  8. 奥　公秀、阪井　康能ミクロオートファジーオートファジー：分子メカニズムの理解から病態の解明まで,第Ⅱ部、第８章(2018).

2017年

  1. Abe M, Sawada Y, Uno S, Chigasaki S, Oku M, Sakai Y, Miyoshi H.Role of Acyl Chain Composition of Phosphatidylcholine in Tafazzin-Mediated Remodeling of Cardiolipin in Liposomes.Biochemistry, 56, 6268-6280 (2017). doi: 10.1021/acs.biochem.7b00941.

  2. Masahide Oku, Yuichiro Maeda, Yoko Kagohashi, Takeshi Kondo, Mai Yamada, Toyoshi Fujimoto, Yasuyoshi Sakai .Evidence for ESCRT- and clathrin-dependent microautophagy. J Cell Biol., 216, 3263 (2017). doi: 10.1083/jcb.201611029.

  3. Katharina Frindte, Marina G. Kalyuzhnaya, Françoise Bringel, Peter F. Dunfield, Mike S. M. Jetten, Valentina N. Khmelenina, Martin G. Klotz, J. Colin Murrell, Huub J. M. Op den Camp, Yasuyoshi Sakai, Jeremy D. Semrau, Nicole Shapiro, Alan A. DiSpirito, Lisa Y. Stein, Mette M. Svenning, Yuri A. Trotsenko, Stéphane Vuilleumier, Tanja Woyke, Claudia Knief.Draft Genome Sequences of Two Gammaproteobacterial Methanotrophs Isolated from Rice Ecosystems.Genome Announc.,5, e00526-17 (2017). doi: 10.1128/genomeA.00526-17.

  4. Yuki Oku, Kazuma Murakami, Kazuhiro Irie, Jun Hoseki, Yasuyoshi SakaiSynthesized Aβ42 Caused Intracellular Oxidative Damage, Leading to Cell Death, via Lysosome Rupture.Cell Struct. Funct. ,42, 71-79 (2017). doi: 10.1247/csf.17006.

  5. Shun-ichi Yamashita, Masahide Oku, Yasuyoshi Sakai, Yukio FujikiExperimental Systems to Study Yeast Pexophagy.Methods Mol Biol., 1595, 249-255 (2017). doi: 10.1007/978-1-4939-6937-1_24.

  6. Ohsawa S, Yurimoto H, Sakai Y.Novel function of Wsc proteins as a methanol-sensing machinery in the yeast Pichia pastoris.Mol Microbiol.,104, 349-363 (2017). doi: 10.1111/mmi.13631.

  7. Yuichiro Maeda, Masahide Oku, and Yasuyoshi SakaiAutophagy-independent function of Atg8 in lipid droplet dynamics in yeastJ. Biochem.,161, 339-348 (2017). doi: 10.1093/jb/mvw078.

  8. Takayuki Ikezoe, Jing Yang, Chie Nishioka, Bin Pan1, Kailin Xu, Mutsuo Furihata, Keigo Nakamura, Hiroya Yurimoto, Yasuyosi Sakai, Goichi Honda, Akihito YokoyamaThe fifth epidermal growth factor-like region of thrombomodulin exerts cytoprotective function and prevents SOS in a murine modelBone Marrow Transplant.,52, 73-79 (2017). doi: 10.1038/bmt.2016.195.

2016年

  1. Shun-ichi Yamashita, Xiulian Jin, Kentaro Furukawa, Maho Hamasaki, Akiko Nezu, Hidenori Otera, Tetsu Saigusa, Tamotsu Yoshimori, Yasuyoshi Sakai, Katsuyoshi Mihara, Tomotake KankiMitochondrial division occurs concurrently with autophagosome formation but independently of Drp1 during mitophagyJ. Cell Biol.,215, 649-665 (2016). doi: 10.1083/jcb.201605093.

  2. 由里本博也メタノール酵母による異種タンパク質生産：高生産のための技術戦略SUNATEC e-Magazine,126, 2 (2016).

  3. Hiroyuki Iguchi, Hiroya Yurimoto and Yasuyoshi SakaiMethylovulum Iguchi, Yurimoto and Sakai 2011, 813VP.Bergey’s Manual of Systematics of Archaea and Bacteria,1-4 (2016). doi: 10.1002/9781118960608.gbm01416.

  4. Abe M, Hasegawa Y, Oku M, Sawada Y, Tanaka E, Sakai Y, Miyoshi H.Mechanism for Remodeling of the Acyl Chain Composition of Cardiolipin Catalyzed by Saccharomyces cerevisiae TafazzinJ Biol Chem. ,291, 15491-15502 (2016). doi: 10.1074/jbc.M116.718510.

  5. 由里本博也、阪井康能生態系の炭素循環を駆動するC1微生物−植物間相互作用生きものたちをつなぐ「かおり」〜エコロジカルボラタイルズ〜（松井健二、高林純示、東原和成 編著),フレグランスジャーナル社, 東京, pp.22-24 (2016).

  6. Masahide Oku, Yasuyoshi SakaiPexophagy in yeastsBiochim Biophys Acta. ,1863(5), 992-998 (2016). doi:10.1016/j.bbamcr.2015.09.023.

  7. Saori Oda, Hiroya Yurimoto, Nobuhisa Nitta, Yasuyoshi SakaiThe unique C-terminal region of Hap3 is required for methanol-regulated gene expression in the methylotrophic yeast Candida boidiniiMicrobiology,162, 898-907 (2016). doi: 10.1099/mic.0.000275.

  8. Jun Hoseki, Asami Oishi, Takaaki Fujimura, Yasuyoshi SakaiDevelopment of a stable ERroGFP variant suitable for monitoring redox dynamics in the ERBiosci. Rep.,36(2), e00316 (2016). doi: 10.1042/BSR20160027.

  9. Sunita Maharjan, Yasuyoshi Sakai, Jun HosekiScreening of dietary antioxidants against mitochondria-mediated oxidative stress by visualization of intracellular redox stateBiosci. Biotech. Biochem.,80(4), 726-234 (2016). doi:10.1080/09168451.2015.1123607.
    [農芸化学会 BBB論文賞]

  10. James D. Flynn, Hisako Hirayama, Yasuyoshi Sakai, Peter F. Dunfield, Martin G. Klotz, Claudia Knief, Huub J. M. Op den Camp, Mike S. M. Jetten, Valentina N. Khmelenina, Yuri A. Trotsenko, J. Colin Murrell, Jeremy D. Semrau, Mette M. Svenning, Lisa Y. Stein, Nikos Kyrpides, Nicole Shapiro, Tanja Woyke, Françoise Bringel, Stéphane Vuilleumier, Alan A. DiSpirito, Marina G. Kalyuzhnaya Draft Genome Sequences of Gammaproteobacterial Methanotrophs Isolated from Marine EcosystemsGenome Announc.,4, e01629-15 (2016).

  11. Jenjira Damtab, Pumin Nutaratat, Wanatchaporn Boontham, Nantana Srisuk, Kannika Duangmal, Hiroya Yurimoto, Yasuyoshi Sakai, Yuki Muramatsu, Yasuyoshi Nakagawa Roseomonas elaeocarpi sp. nov., isolated from olive (Elaeocarpus hygrophilus Kurz.) phyllosphereInt. J. Syst. Evol. Microbiol.,66, 474-480 (2016)

2015年

  1. 由里本博也、阪井康能C1微生物の巧妙なストレス適応機構と多様な生育環境での生存戦略極限環境生物学会誌,14(2), 54-62 (2015).

  2. Kosuke Shiraishi, Masahide Oku, Daichi Uchida, Hiroya Yurimoto, Yasuyoshi SakaiRegulation of nitrate and methylamine metabolism by multiple nitrogen sources in the methylotrophic yeast Candida boidiniiFEMS Yeast Res.,15, fov084 (2015).

  3. 由里本博也、阪井康能C1微生物-植物共生系によるC1炭素固定と植物生長促進光合成研究,25(2), 92-99 (2015).

  4. 井口博之、由里本博也、阪井康能第15章 C1微生物と植物の共生難培養微生物研究の最新技術III―微生物の生き様に迫り課題解決へ―（大熊盛也、野田悟子監修),シーエムシー出版, 東京, pp.135-144 (2015).

  5. 寳関　淳、阪井康能プロテアソーム阻害が引き起こすレドックス異常と抗酸化食品による制御バイオサイエンスとインダストリー,73(3), 198-201 (2015).

  6. 寳関　淳、阪井康能細胞内レドックス状態の可視化によるレドックスモジュレーターの探索バイオサイエンスとインダストリー,73(3), 192-193 (2015).

  7. Yuichiro Maeda, Masahide Oku, and Yasuyoshi SakaiA defect of the vacuolar putative lipase Atg15 accelerates degradation of lipid droplets through lipolysisAutophagy,11, 1247-1258 (2015).

  8. Peter Dunfield, Christine Sharp, Angela Smirnova, Marina Kalyuzhnaya, Françoise Bringel, Hisako Hirayama, Mike Jetten, Valentina Khmelenina, Martin Klotz, Claudia Knief, Nikos Kyrpides, Huub Op den Camp, Aleksandr Reshetnikov, Yasuyoshi Sakai, Nicole Shapiro, Yuri Trotsenko, Stéphane Vuilleumier, and Tanja WoykeDraft genome sequence of the moderately halophilic methanotroph, Methylohalobius crimeensis strain 10KiGenome Announc.,3, e00644-15 (2015).

  9. Richard Hamilton, K. Dimitri Kits, Victoria A. Ramonovskaya, Olga N. Rozova, Hiroya Yurimoto, Hiroyuki Iguchi, Valentina N. Khmelenina, Yasuyoshi Sakai, Peter F. Dunfield, Martin G. Klotz, Claudia Knief, Huub J. M. Op den Camp, Mike S. M. Jetten, Françoise Bringel, Stéphane Vuilleumier, Mette M. Svenning, Nicole Shapiro, Tanja Woyke, Yuri A. Trotsenko, Lisa Y. Stein, Marina G. KalyuzhnayaDraft Genomes of Gammaproteobacterial Methanotrophs Isolated from Terrestrial EcosystemsGenome Announc.,3, e00515-15 (2015).

  10. Kosuke Shiraishi, Masahide Oku, Kosuke Kawaguchi, Daichi Uchida, Hiroya Yurimoto and Yasuyoshi SakaiYeast nitrogen utilization in the phyllosphere during plant lifespan under regulation of autophagyScientific Reports,5, 9719 (2015).

  11. Hiroyuki Iguchi, Hiroya Yurimoto and Yasuyoshi Sakai * Interactions of Methylotrophs with Plants and Other Heterotrophic BacteriaMicroorganisms,3(2), 137-151 (2015).

  12. Oda S, Yurimoto H, Nitta N, Sasano Y, Sakai Y.Molecular characterization of Hap complex components responsible for methanol-inducible gene expression in the methylotrophic yeast Candida boidinii.Eukaryot Cell.,14, 278-285 (2015).

2014年

  1. Jun Hoseki and Yasuyoshi Sakai Anti-aging Compounds Act in MitochondriaKyoto University Research Activities,4(3), 26 (2014).4, Article number: 5896 doi:10.1038/srep05896.

  2. 奥 公秀、田村直輝、阪井康能メチロトロフ酵母における多様なオートファジー経路とその生理機能化学と生物,52(12), 825-829 (2014).

  3. Sunita Maharjan, Masahide Oku, Masashi Tsuda, Jun Hoseki and Yasuyoshi Sakai Mitochondrial impairment triggers cytosolic oxidative stress and cell death following proteasome inhibitionSci. Rep.,4, 5896 (2014).
    [京大HPにて紹介]　[朝日新聞（8月1日 4面）に紹介記事]　[京都新聞（8月1日 26面）に紹介記事]　[産経新聞（8月1日 26面）に紹介記事]　[日刊工業新聞（8月1日 23面）に紹介記事]　[毎日新聞（8月1日 23面）に紹介記事]　[他、多数の全国地方紙に紹介記事]

  4. Wanatchabhorn Boontham, Nantana Srisuk, Kanya Kokaew, Pantida Treeyoung, Savitree Limtong, Arinthip Thamchaipenet and Hiroya Yurimoto Xylitol production by thermotolerant methylotrophic yeast Ogataea siamensis and its xylose reductase gene (XYL1) cloningChiang Mai J. Sci.,41(3), 491-502 (2014).

  5. Masamune Aihara, Xiulian Jin, Yusuke Kurihara, Yutaka Yoshida, Yuichi Matsushima, Masahide Oku, Yuko Hirota, Tetsu Saigusa, Yoshimasa Aoki, Takeshi Uchiumi, Tadashi Yamamoto, Yasuyoshi Sakai, Dongchon Kang, and Tomotake Kanki. The Tor and Sin3-Rpd3 complex regulate expression of the mitophagy receptor protein Atg32J. Cell. Sci.,127, 3184-3196 (2014).

  6. S. Hoefman, D. van der Ha, H. Iguchi, H. Yurimoto, Y. Sakai, N. Boon, P. Vandamme, K. Heylen, and P. de Vos.Methyloparacoccus murrellii gen. nov., sp. nov., a novel methanotroph isolated from pond water in South Africa and Japan.Int. J. Syst. Evol. Microbiol.,64, 2100-2107 (2014).

  7. 由里本博也、阪井康能植物から放出されるC1化合物と微生物―植物間相互作用による炭素循環AROMA RESEARCH,15(1), 80-84 (2014).

  8. M. Oku, Y. Takanno, and Y. Sakai.The emerging role of autophagy in peroxisome dynamics and lipid metabolism of phyllosphere microorganisms.Front. Plant Sci.,5, 81 (2014).

  9. 寳関　淳、奥　公秀、阪井康能蛍光タンパク質プローブを用いた細胞内レドックス状態の可視化計測バイオサイエンスとインダストリー,72(1), 8-13 (2014).

  10. N. Yoshida, H. Iguchi, H. Yurimoto, A. Murakami, and Y. Sakai.Aquatic plant surface as a niche for methanotrophs.Front. Microbiol., 5, 30 (2014).
    [Top10 most viewed Microbiology research articles in February 2014.]

  11. N. Tamura, M. Oku, and Y. Sakai.Atg21 regulates pexophagy via its PI(3)P binding activity in Pichia pastoris.FEMS Yeast Res.,14, 435–444 (2014).
    [Fig.5c: Selected as the annual cover image for FEMS Yeast Research 2014 issues.]

  12. K. Kawaguchi, H. Yurimoto, and Y. Sakai.Expression of a codon-optimized Aspergillus niger pectin methylesterase gene in the methylotrophic yeast Candida boidinii.Biosci. Biotechnol. Biochem.,78, 718-721 (2014).

  13. C. Li, A. Kita, Y. Hashimoto, M. Ihara, A. Kato, N. Ogura, A. Doi, M. Oku, T. Itoh, Y. Sakai, and R. Sugiura.The functional link between Rab GTPase-mediated membrane trafficking and PI4,5P2 signaling.Genes Cells,19, 177-197 (2014).

2013年

  1. 阪井康能、由里本博也葉面に棲息するC1微生物：共生系による炭素循環と環境バイオへの利用環境バイオテクノロジー学会誌,13(2), 79-84 (2013).

  2. M. Oku, N. Tamura, and Y. Sakai.Atg18 lifts up from and lands on the vacuolar membrane mediated by phosphorylation of its propellers.Autophagy,9, 2161-2162 (2013).

  3. H. Iguchi, I. Sato, H. Yurimoto, and Y. Sakai.Stress resistance and C1 metabolism involved in plant colonization of a methanotroph Methylosinus sp. B4S.Arch. Microbiol.,195, 717-726 (2013).

  4. 由里本博也、井口博之、阪井康能C1微生物複合生物系が駆動する炭素循環（メタンサイクル）生物の科学遺伝,67(5), 591-595 (2013).

  5. N. Tamura, M. Oku, M. Ito, N. Noda, F. Inagaki, and Y. Sakai.Atg18 phosphoregulation controls organellar dynamics by modulating its phosphoinositide-binding activity.J. Cell Biol.,202, 685-698 (2013).
    [京大HPにて紹介]　[掲載誌 In Focusに紹介記事]　 [Selected as Faculty 1000 Prime Article]　 [京都新聞に紹介記事]　[化学 に紹介記事]

  6. 阪井康能、由里本博也、奥　公秀オルガネラタンパク質の輸送・分解の分子基盤と有用タンパク質生産　－メタノール資化性酵母の発達したペルオキシソームの利用酵母の生命科学と生物工学　産業応用から基礎科学へ （原島俊、高木博史編),化学同人, 京都, pp.151-165 (2013).

  7. 由里本博也、阪井康能C1微生物代謝経路の省エネ型炭素固定系としての利用とその問題点生物工学会誌,91(7), 384-387 (2013).

  8. M. Oku, Y. Ichiki, A. Shiraishi, T. Takahara, T. Maeda, and Y. Sakai.Hyper-activation of target of rapamycin (TOR) kinase 1 decreases intracellular glutathione content in Saccharomyces cerevisiae revealed by LC-MS/MS analysis.Biosci. Biotechnol. Biochem.,77, 1608-1611 (2013).

  9. V. Khmelenina, D. Beck, C. Munk, K. Davenport, H. Daligault, T. Erkkila, L. Goodwin, W. Gu, C. Lo, M. Scholz, H. Teshima, Y. Xu, P. Chain, F. Bringel, S. Vuilleumier, A. DiSpirito, P. Dunfield, M. Jetten, M. Klotz, C. Knief, C. Murrell, H. Op den Camp, Y. Sakai, J. Semrau, M. Svenning, L. Stein, Y. Trotsenko, and M. Kalyuzhnaya.Draft genome sequence of Methylomicrobium buryatense 5G, a haloalkaline-tolerant methanotrophic bacterium.Genome Announc.,1, e00053-13 (2013).

  10. M. Mizuno, H. Yurimoto, H. Iguchi, A. Tani, and Y. Sakai.Dominant colonization and inheritance of Methylobacterium sp. strain OR01 on perilla plants.Biosci. Biotechnol. Biochem.,77, 1533-1538 (2013).

  11. S. Fujimura, H. Yurimoto, S. Kurimoto, Y. Matsufuji, T. Ito, T. Hayakawa, N. Tomizuka, Y. Sakai, and T. Nakagawa.Expression level of methanol-inducible peroxisomal proteins and peroxisome morphology are affected by oxygen conditions and mitochondrial respiratory pathway function in the methylotrophic yeast Candida boidinii.FEMS Yeast Res.,13, 359-366 (2013).

  12. M. Oku, J. Hoseki, Y. Ichiki, and Y. Sakai.A fluorescence resonance energy transfer (FRET)-based redox sensor reveals physiological role of thioredoxin in the yeast Saccharomyces cerevisiae.FEBS Lett.,587, 793-798 (2013).

  13. 寳関　淳、奥　公秀、阪井康能酵母のレドックス認識機構を応用した新しいセンサータンパク質の開発FRAGRANCE JOURNAL,41(2), 64-69 (2013).

  14. P. Nutaratat, N. Srisuk, K. Duangmal, H. Yurimoto, Y. Sakai, Y. Muramatsu, and Y. Nakagawa.Roseomonas musae sp. nov., a new bacterium isolated from a banana phyllosphere.Antonie van Leeuwenhoek,103, 617-624 (2013).

2012年

  1. 阪井康能C1酵母の植物葉上でのライフスタイル科研費NEWS,2012年度Vol. 3, 17 (2012).

  2. M. Asakura, K. Yoshino, A.M.. Hill, Y. Kubo, Y. Sakai, and Y. Takano.Primary and secondary metabolism regulates lipolysis in appressoria of Colletotrichum orbiculare.Fungal Genet. Biol,49, 967-975 (2012).

  3. 阪井康能、寳関　淳、奥　公秀レドックス異常を回復する化合物レドックスモジュレーターの探索：Redoxfluorの創薬への利用遺伝子医学MOOK,22, 158-163 (2012).

  4. 奥　公秀、阪井康能オートファジーによるペルオキシソームの選択的分解と生理機能生体の科学,63(5), 500-501 (2012).

  5. Z. Zhai, H. Yurimoto, and Y. Sakai.Molecular characterization of the Candida boidinii MIG1 and its role in regulation of methanol-inducible gene expression.Yeast,29, 293-301 (2012).

  6. H. Iguchi, I. Sato, M. Sakakibara, H. Yurimoto, and Y. Sakai.Distribution of methanotrophs in the phyllosphere.Biosci. Biotechnol. Biochem.,76, 1580-1583 (2012).

  7. M. Mizuno, H. Yurimoto, N. Yoshida, H. Iguchi, and Y. Sakai.Distribution of pink-pigmented facultative methylotrophs on leaves of vegetables.Biosci. Biotechnol. Biochem.,76, 578-580 (2012).

  8. Z. Zhai, H. Yurimoto, and Y. Sakai.Msn5p is involved in formaldehyde resistance but not in oxidative stress response in the methylotrophic yeast Candida boidinii.Biosci. Biotechnol. Biochem.,76, 299-304 (2012).

  9. M. Oku and Y. Sakai.Assessment of physiological redox state with FRET protein probes.Antioxid. Redox Signal.,16, 698-704 (2012).

2011年

  1. H. Iguchi, H. Yurimoto, and Y. Sakai.Stimulation of methanotrophic growth in co-cultures by cobalamin excreted by rhizobia.Appl. Environ. Microbiol.,77, 8509-8515 (2011).

  2. K. Kawaguchi, H. Yurimoto, M. Oku, and Y. Sakai.Yeast methylotrophy and autophagy in a methanol-oscillating environment on growing Arabidopsis thaliana leaves.PLoS One,6, e25257 (2011).
    [京都新聞 に紹介記事]　[蛍雪時代 に紹介記事]　[京大HP にて紹介]

  3. 奥　公秀、阪井康能細胞内の酸化還元状態を検知するタンパク質FRETプローブ, レドックスフロール　酵母の酸化ストレス応答から学び, それを利用する新戦略化学と生物,49(8), 516-517 (2011).

  4. 阪井康能、由里本博也C1微生物複合生物系による省エネ型炭素固定技術環境資源工学,58(2), 59-63 (2011).

  5. C. Nishizaki, M. Nishikawa, T. Yata, T. Yamada, Y. Takahashi, M. Oku, H. Yurimoto, Y. Sakai, K. Nakanishi, and Y. Takakura.Inhibition of surgical trauma-enhanced peritoneal dissemination of tumor cells by human catalase derivatives in mice.Free Radic. Biol. Med.,51, 773-779 (2011).

  6. H. Yurimoto, M. Oku, and Y. Sakai.Yeast methylotrophy: metabolism, gene regulation, and peroxisome homeostasis.Int. J. Microbiol.,2011, 101298 (2011).

  7. H. Iguchi, H. Yurimoto, and Y. Sakai.Methylovulum miyakonense gen. nov., sp. nov., a type I methanotroph from forest soil.Int. J. Syst. Evol. Microbiol.,61, 810-815 (2011).

  8. Y. Fukuta, S. Nanda, Y. Kato, H. Yurimoto, Y. Sakai, H. Komeda, and Y. Asano.Characterization of a new (R)-hydroxynitrile lyase from the Japanese apricot Prunus mume and cDNA cloning and secretory expression of one of the isozymes in Pichia pastoris.Biosci. Biotechnol. Biochem.,75, 214-220 (2011).

  9. 奥　公秀、阪井康能Redoxfluorを用いた細胞内酸化還元状態の可視化実験医学,29(6), 945-950 (2011).

  10. 由里本博也、阪井康能光合成経路と相性のよいホルムアルデヒド固定反応バイオサイエンスとインダストリー,69(1), 46-48 (2011).

2010年

  1. 由里本博也、阪井康能C1微生物と植物の相互作用と炭素循環　－代謝生化学・分子細胞生物学からの理解－植物の生長調節,45(2), 125-131 (2010).

  2. N. Tamura, M. Oku, and Y. Sakai.Atg8 regulates vacuolar membrane dynamics in a lipidation-independent manner in Pichia pastoris.J. Cell Sci.,123, 4107-4116 (2010).

    [掲載誌 In This Issueに紹介記事]

  3. I. Orita, A. Kita, H. Yurimoto, N. Kato, Y. Sakai, and K. Miki.Crystal structure of 3-hexulose-6-phosphate synthase, a member of the orotidine 5′-monophosphate decarboxylase suprafamily.Proteins,78, 3488-3492 (2010).

  4. H. Iguchi, H. Yurimoto, and Y. Sakai.Soluble and particulate methane monooxygenase gene clusters in the type I methanotroph Methylovulum miyakonense HT12.FEMS Microbiol. Lett.,312, 71-76 (2010).

  5. Z. Song, I. Orita, F. Yin, H. Yurimoto, N. Kato, Y. Sakai, K. Izui, K. Li, and L. Chen.Overexpression of an HPS/PHI fusion enzyme from Mycobacterium gastri in chloroplasts of geranium enhances its ability to assimilate and phytoremediate formaldehyde.Biotechnol. Lett.,32, 1541-1548 (2010).

  6. M. Oku and Y. Sakai.Peroxisomes as dynamic organelles: autophagic degradation.FEBS J.,277, 3289-3294 (2010).

  7. T. Yano, M. Oku, N. Akeyama, A. Itoyama, H. Yurimoto, S. Kuge, Y. Fujiki, and Y. Sakai.A novel fluorescent sensor protein for visualization of redox states in the cytoplasm and in peroxisomes.Mol. Cell. Biol.,30, 3758-3766 (2010).
    [Most read article Jul-Sep　2010 ランクイン]　[京都新聞 に紹介記事]　[朝日新聞、京都新聞、毎日新聞、読売新聞 に紹介記事]　[京大HP にて紹介]

  8. T. Nakagawa, K. Yoshida, A. Takeuchi, T. Ito, S. Fujimura, Y. Matsufujii, N. Tomizuka, H. Yurimoto, Y. Sakai, and T. Hayakawa.Peroxisomal catalase gene in the methylotrophic yeast Pichia methanolica.Biosci. Biotechnol. Biochem.,74, 1733-1735 (2010).

  9. T. Nakagawa, S. Fujimura, T. Ito, Y. Matsufujii, S. Ozawa, T. Miyaji, J. Nakagawa, N. Tomizuka, H. Yurimoto, Y. Sakai, and T. Hayakawa.Molecular characterization of two genes with high similarity to the dihydroxyacetone synthase gene in the methylotrophic yeast Pichia methanolica.Biosci. Biotechnol. Biochem.,74, 1491-1493 (2010).

  10. Y. Sasano, H. Yurimoto, M. Kuriyama, and Y. Sakai.Trm2p-dependent derepression is essential for methanol-specific gene activation in the methylotrophic yeast Candida boidinii.FEMS Yeast Res.,10, 535-544 (2010).

  11. 阪井康能自然界での生存戦略からみたオートファジーと糸状菌の植物病原性～To be, or not to be: that is the question・・・・～化学と生物,48(3), 153-155 (2010).

  12. K. Yoshimoto, Y. Takano, and Y. Sakai.Autophagy in plants and phytopathogens.FEBS Lett.,584, 1350-1358 (2010).

  13. L. M. Chen, H. Yurimoto, K. Z. Li, I. Orita, M. Akita, N. Kato, Y. Sakai, and K. Izui.Assimilation of formaldehyde in transgenic plants due to the introduction of the bacterial ribulose monophosphate pathway genes.Biosci. Biotechnol. Biochem.,74, 627-635 (2010).
    [農芸化学会 BBB論文賞]　[朝日新聞　朝刊一面に紹介記事、他報道多数]

2009年

  1. T. Yata, M. Nishikawa, C. Nishizaki, M. Oku, H. Yurimoto, Y. Sakai, and Y. Takakura.Control of hypoxia-induced tumor cell adhesion by cytophilic human catalase.Free Radic. Biol. Med.,47, 1772-1778 (2009).

  2. 阪井康能、高野義孝植物病原菌の宿主感染・オルガネラ数の制御とオートファジーブレインテクノニュース,135, 25-30 (2009).

  3. H. Yurimoto, N. Kato, and Y. Sakai.Genomic organization and biochemistry of the ribulose monophosphate pathway and its application in biotechnology.Appl. Microbiol. Biotechnol.,84, 407-416 (2009).

  4. Y. Takano, M. Asakura, and Y. Sakai.Atg26-mediated pexophagy and fungal phytopathogenicity.Autophagy,5, 1041-1042 (2009).

  5. Y. Watanabe, N.N. Noda, K. Honbou, K. Suzuki, Y. Sakai, Y. Ohsumi, and F. Inagaki.Crystallization of Saccharomyces cerevisiae alpha-mannosidase, a cargo protein of the Cvt pathway.Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun.,65, 571-573 (2009).

  6. S. Yamashita, H. Yurimoto, D. Murakami, M. Yoshikawa, M. Oku, and Y. Sakai.Lag-phase autophagy in the methylotrophic yeast Pichia pastoris.Genes Cells,14, 861-870 (2009).

  7. T. Yano, H. Yurimoto, and Y. Sakai.Activation of the oxidative stress regulator PpYap1 through conserved cysteine residues during methanol metabolism in the yeast Pichia pastoris.Biosci. Biotechnol. Biochem.,73, 1404-1411 (2009).

  8. M. Asakura, S. Ninomiya, M. Sugimoto, M. Oku, S. Yamashita, T. Okuno, Y. Sakai, and Y. Takano.Atg26-mediated pexophagy is required for host invasion by the plant pathogenic fungus Colletotrichum orbiculare.Plant Cell,21, 1291-1304 (2009).
    [京大HPに掲載]　[掲載誌　In Brief に紹介記事]　[京都新聞、日本工業新聞に紹介記事]

  9. T. Yano, E. Takigami, H. Yurimoto, and Y. Sakai.The Yap-1 regulated glutathione redox system curtails the accumulation of formaldehyde and reactive oxygen species in methanol metabolism of Pichia pastoris.Eukaryot. Cell,8, 540-549 (2009).

  10. H. Yurimoto and Y. Sakai.Methanol-inducible gene expression and heterologous protein production in the methylotrophic yeast Candida boidinii.Biotechnol. Appl. Biochem.,53, 85-92 (2009).

  11. H. Yurimoto.Molecular basis of methanol-inducible gene expression and its application in the methylotrophic yeast Candida boidinii.Biosci. Biotechnol. Biochem.,73, 793-800 (2009).

  12. K. Egawa, H. Shibata, S. Yamashita, H. Yurimoto, Y. Sakai, and H. Kato.Overexpression and purification of rat peroxisomal membrane protein 22, PMP22, in Pichia pastoris.Protein Expr. Purif.,64, 47-54 (2009).

2008年

  1. M. Oku and Y. Sakai.Pexphagy in Pichia pastoris.Methods Enzymol.,451, 217-228 (2008).

  2. 由里本博也、折田和泉、阪井康能アーキアにおけるホルムアルデヒド固定酵素群の生理機能バイオサイエンスとインダストリー,66(8), 447-449 (2008).

  3. 由里本博也、阪井康能微生物による組換えタンパク質生産タンパク質の事典 （猪飼篤、伏見譲、卜部格、上野川修一、中村春木、浜窪隆雄編), 朝倉書店, 東京,pp.726-728 (2008).

  4. 由里本博也、阪井康能微生物のしくみ生物資源から考える21世紀の農学 第6巻 微生物機能の開発 （植田充美編), 京都大学学術出版会, 京都,pp.3-27 (2008).

  5. 阪井康能オルガネラ・ダイナミクスとオートファジー実験医学,26(2), 284－288 (2008).

  6. 熊谷英彦、加藤暢夫、村田幸作、阪井康能　編著遺伝子から見た応用微生物学朝倉書店, 東京,-2008.

  7. Y. Sasano, H. Yurimoto, M. Yanaka, and Y. Sakai.Trm1p, a Zn(II)2Cys6-type transcription factor, is a master regulator of methanol-specific gene activation in the methylotrophic yeast Candida boidinii.Eukaryot. Cell,7, 527-536 (2008).

  8. S. Limtong, N. Srisuk, W. Yongmanitchai, H. Yurimoto, and T. Nakase.Ogataea chonburiensis sp. nov. and Ogataea nakhonphanomensis sp. nov., two thermotolerant, methylotrophic yeast species isolated in Thailand and the transfer of Pichia siamensis and Pichia thermomethanolica to the genus Ogataea.Int. J. Syst. Evol. Microbiol.,58, 302-307 (2008).

2007年

  1. A. Sawada, T. Oyabu, L.M. Chen, K.Z. Li, H. Yurimoto, I. Orita, Y. Sakai, N. Kato, and K. Izui.Purification capability of tobacco transformed with enzymes from a methylotrophic bacterium for formaldehyde.Int. J. Phytoremediation,9, 487-496 (2007).

  2. 由里本博也、阪井康能異種タンパク質生産酵母のすべて （大隅良典・下田親編), シュプリンガー・ジャパン, 東京,pp.315-319 (2007).

  3. 阪井康能ペルオキシソーム酵母のすべて （大隅良典・下田親編), シュプリンガー・ジャパン, 東京,pp.83-86 （2007).

  4. I. Orita, N. Sakamoto, N. Kato, H. Yurimoto, and Y. Sakai.Bifunctional enzyme fusion of 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerase.Appl. Microbiol. Biotechnol.,76, 439-445 (2007).

  5. Y. Sasano, H. Yurimoto, and Y. Sakai.Gene-tagging mutagenesis in the methylotrophic yeast Candida boidinii.J. Biosci. Bioeng.,104, 86-89 (2007).

  6. T. Kotani, H. Yurimoto, N. Kato, and Y. Sakai.A novel acetone metabolism in a propane-utilizing bacterium Gordonia sp. strain TY-5.J. Bacteriol.,189, 886-893 (2007).

  7. S. Yamashita, M. Oku, and Y. Sakai.Functions of PI4P and sterol glucoside necessary for the synthesis of a nascent membrane structure during pexophagy.Autophagy,3, 35-37 (2007).

  8. 由里本博也、阪井康能未来型資源の利用を目指すC1微生物化学工業,58(1), 21-26 (2007).

2006年

  1. H. YurimotoMolecular mechanism of strategy for minimization of the intracellular formaldehyde level in microorganisms and its application.Report on the Noda Institute for Scientific Research,50, 77-78 (2006).

  2. M. Oku, T. Nishimura, T. Hattori, Y. Ano, S. Yamashita, and Y. Sakai.Role of Vac8 in formation of the vacuolar sequestering membrane during micropexophagy.Autophagy,2, 272-279 (2006).

  3. 阪井康能酵母ミクロペキソファジーの細胞内ダイナミクス顕微鏡,41(2), 81-83 (2006).

  4. 山下俊一、阪井康能Atg分子による膜動態の制御とペキソファジー蛋白質・核酸・酵素,51(10), 1474-1479 (2006).

  5. 阪井康能、奥　公秀、向山博幸微生物の観察実験化学講座 29 バイオテクノロジーの基本技術 (日本化学会編), 丸善, 東京,19-33（2006).

  6. 加藤暢夫、由里本博也微生物の分離実験化学講座 29 バイオテクノロジーの基本技術 (日本化学会編), 丸善, 東京,1-18（2006).

  7. Y. Sakai, M. Oku, I.J. van der Klei, and J.A.K.W. Kiel.Pexophagy: autophagic degradation of peroxisomes.Biochim. Biophys. Acta,1763, 1767-1775 (2006).

  8. I.J. van der Klei, H. Yurimoto, Y. Sakai, and M. Veenhuis.The significance of peroxisomes in methanol metabolism in methylotrophic yeast.Biochim. Biophys. Acta,1763, 1453-1462 (2006).

  9. T. Kotani, Y. Kawashima, H. Yurimoto, N. Kato, and Y. Sakai.Gene Structure and Regulation of Alkane Monooxygenases in Propane-utilizing Mycobacterium sp. TY-6 and Pseudonocardia sp. TY-7.J. Biosci. Bioeng.,102, 184-192 (2006).

  10. S. Yamashita, M. Oku, Y. Wasada, Y. Ano, and Y. Sakai.PI4P-signaling pathway for the synthesis of a nascent membrane structure in selective autophagy.J. Cell Biol.,173, 709-717 (2006).

  11. I. Orita, T. Sato, H. Yurimoto, N. Kato, H. Atomi, T. Imanaka, and Y. Sakai.The Ribulose Monophosphate Pathway Substitutes for the Missing Pentose Phosphate Pathway in the Archaeon Thermococcus kodakaraensis.J. Bacteriol.,188, 4698-4704 (2006).

  12. K. Kawaguchi, Y. Shinoda, H. Yurimoto, Y. Sakai, and N. Kato.Purification and characterization of benzoate-CoA ligase from Magnetospirillum sp. strain TS-6 capable of aerobic and anaerobic degradation of aromatic compounds.FEMS Microbiol. Lett.,257, 208-213 (2006).

  13. M. Kajikawa, K. T. Yamato, Y. Kohzu, S. Shoji, K. Matsui, Y. Tanaka, Y. Sakai, and H. Fukuzawa.A front-end desaturase from Chlamydomonas reinhardtii produces pinolenic and coniferonic acids by (omega)13 desaturation in methylotrophic yeast and Tobacco.Plant Cell Physiol.,47, 64-73 (2006).

  14. M. Kajikawa, K. T. Yamato, Y. Sakai, H. Fukuzawa, K. Ohyama, and T. Kohchi.Isolation and functional characterization of fatty acid (delta)5-elongase gene from the liverwort Marchantia polymorpha L..FEBS Lett.,580, 149-154 (2006).

  15. T. Nakagawa, A. Inagaki, T. Ito, S. Fujimura, T. Miyaji, H. Yurimoto, N. Kato, Y. Sakai, and N. Tomizuka.Regulation of two distinct alcohol oxidase promoters in the methylotrophic yeast Pichia methanolica.Yeast,23, 15-22 (2006).

  16. N. Kato, H. Yurimoto, and R. K. Thauer.The physiological role of the ribulose monophosphate pathway in bacteria and archaea.Biosci. Biotechnol. Biochem.,70, 10-21 (2006).

2005年

  1. 由里本博也、加藤暢夫、阪井康能酵母、細菌、アーキアのC1化合物代謝バイオサイエンスとインダストリー,63(12), 773-776 (2005).

  2. 阪井康能未来型資源の利用を目指すC1微生物の理解バイオサイエンスとインダストリー,63(10), 643-645 (2005).

  3. T. Kotani, H. Yurimoto, N. Kato, and Y. Sakai.Purification and characterization of three NAD(+)-dependent secondary alcohol dehydrogenases from propane-utilizing bacterium Gordonia sp. strain TY-5.応用微生物学研究,3(1), 135-148 (2005).

  4. W. A. Dunn, Jr., J. M. Cregg, J. A. K. W. Kiel, I. J. van der Klei, M. Oku, Y. Sakai, A. A. Sibirny, O. A. Stasyk, and M. Veenhuis.Pexophagy: The Selective Autophagy of PeroxisomesAutophagy,1, 75-83 (2005).

  5. H. Yurimoto, N. Kato, and Y. Sakai.Assimilation, dissimilation, and detoxification of formaldehyde, a central metabolic intermediate of methylotrophic metabolism.Chem. Rec.,5, 367-375 (2005).

  6. M. Kajikawa, K. T. Yamato, H. Fukuzawa, Y. Sakai, H. Uchida, and K. Ohyama.Cloning and characterization of a cDNA encoding beta-amyrin synthase from petroleum plant Euphorbia tirucalli L.Phytochemistry,66, 1759-1766 (2005).

  7. 阪井康能異種タンパク質生産におけるタンパク質分解系とその回避への戦略生物工学ハンドブック (日本生物工学会編), コロナ社, 東京,136-138（2005).

  8. M. Goenrich, R. K. Thauer, H. Yurimoto, and N. Kato.Formaldehyde activating enzyme (Fae) and hexulose-6-phosphate synthase (Hps) in Methanosarcina barkeri: a possible function in ribose-5-phosphate biosynthesis.Arch. Microbiol.,184, 41-48 (2005).

  9. Y. Ano, T. Hattori, N. Kato, and Y. Sakai.Intracellular ATP correlates with mode of pexophagy in Pichia pastoris.Biosci. Biotechnol. Biochem.,69, 1527-1533 (2005).

  10. Y. Shinoda, J. Akagi, Y. Uchihashi, A. Hiraishi, H. Yukawa, H. Yurimoto, Y. Sakai, and N. Kato.Anaerobic degradation of aromatic compounds by Magnetospirillum strains: isolation and degradation genes.Biosci. Biotechnol. Biochem.,69, 1483-1491 (2005).

  11. S. Limtong, N. Srisuk, W. Yongmanitchai, H. Yurimoto, T. Nakase, and N. Kato.Pichia thermomethanolica sp. nov., a new thermotolerant methylotrophic yeast isolated in Thailand.Int. J. Syst. Evol. Microbiol.,55, 2225-2229 (2005).

  12. H. Yurimoto, R. Hirai, N. Matsuno, H. Yasueda, N. Kato, and Y. Sakai.HxlR, a member of the DUF24 protein family, is a DNA-binding protein that acts as a positive regulator of the formaldehyde-inducible hxlAB operon in Bacillus subtilis.Mol. Microbiol.,57, 511-519 (2005).

  13. I. Orita, H. Yurimoto, R. Hirai, Y. Kawarabayasi, Y. Sakai, and N. Kato.The archaeon Pyrococcus horikoshii possesses a bifunctional enzyme for formaldehyde fixation via the ribulose monophosphate pathway.J. Bacteriol.,187, 3636-3642 (2005).

  14. 篠田吉史、阪井康能、加藤暢夫脱窒性細菌による芳香族化合物の嫌気分解－微生物代謝の新たなフロンティア－用水と排水,47(3), 182-189 (2005).

  15. Y. Ano, T. Hattori, M. Oku, H. Mukaiyama, M. Baba, Y. Ohsumi, N. Kato, and Y. Sakai.A sorting nexin PpAtg24 regulates vacuolar membrane dynamics during pexophagy via binding to phosphatidylinositol-3-phosphate.Mol. Biol. Cell,16, 446-457 (2005).

2004年

  1. 米田俊浩、玉井幸夫、近藤恵二、由里本博也、加藤暢夫、阪井康能ギ酸誘導性遺伝子発現機構の解明とメタノール資化性酵母異種タンパク質生産系の確立応用微生物学研究,2(2), 75-81 (2004).

  2. 阪井康能メタノール資化性酵母遺伝子発現系を用いた有用酵素生産の最適化生物工学会誌,82(11), 520-522 (2004).

  3. H. Yurimoto, M. Yamane, Y. Kikuchi, H. Matsui, N. Kato, and Y. Sakai.The pro-peptide of Streptomyces mobaraensis transglutaminase functions in cis and in trans to mediate efficient secretion of active enzyme from methylotrophic yeasts.Biosci. Biotechnol. Biochem.,68, 2058-2069 (2004).[農芸化学会 BBB論文賞]

  4. Y. Sakai, H. Takahashi, Y, Wakasa, T. Kotani, H. Yurimoto, N. Miyachi, P. P. Van Veldhoven, and N. Kato.Role of {alpha}-methylacyl coenzyme A racemase in the degradation of methyl-branched alkanes by Mycobacterium sp. strain P101.J. Bacteriol.,186, 7214-7220 (2004).

  5. S. Limtong, N. Srisuk, W. Yongmanitchai, H. Kawasaki, H. Yurimoto, T. Nakase, and N. Kato.Three new thermotolerant methylotrophic yeasts, Candida krabiensis sp. nov., Candida sithepensis sp. nov., and Pichia siamensis sp. nov., isolated in Thailand.J. Gen. Appl. Microbiol.,50, 119-127 (2004).

  6. 加藤暢夫微生物の新規な代謝機能の解明とその応用に関する研究日本農芸化学会誌,78(6), 548-555 (2004).

  7. 加藤暢夫、石毛たける、阪井康能ノルマルパラフィンからのワックス生産科学と工業,78(6), 302-309 (2004).

  8. 阪井康能、由里本博也メタノール資化性酵母Pichia pastorisのゲノム解析ゲノミクス・プロテオミクスの新展開 (今中忠行監修), エヌ・ティー・エス, 東京,pp.215-220 (2004).

  9. M. Kajikawa, K. T. Yamato, Y. Kohzu, M. Nojiri, E. Sakuradani, S. Shimizu, Y. Sakai, H. Fukuzawa, and K. Ohyama.Isolation and characterization of delta(6)-desaturase, an ELO-like enzyme and delta(5)-desaturase from the liverwort Marchantia polymorpha and production of arachidonic and eicosapentaenoic acids in the methylotrophic yeast Pichia pastoris.Plant Mol. Biol.,54, 335-352 (2004).

  10. T. Nakagawa, T. Ito, S. Fujimura, M. Chikui, T. Mizumura, T. Miyaji, H. Yurimoto, N. Kato, Y. Sakai, and N. Tomizuka.Molecular characterization of glutathione-dependent formaldehyde dehydrogenase gene FLD1 from methylotrophic yeast Pichia methanolica.Yeast,21, 445-453 (2004).

  11. Y. Shinoda, Y. Sakai, H. Uenishi, Y. Uchihashi, A. Hiraishi, H. Yukawa, H. Yurimoto, and N. Kato.Aerobic and anaerobic toluene degradation by a newly isolated denitrifying bacterium, Thauera sp. strain DNT-1.Appl. Environ. Microbiol.,70, 1385-1392 (2004).

  12. H. Yurimoto, B. Lee, F. Yasuda, Y. Sakai, and N. Kato.Alcohol dehydrogenases that catalyse methyl formate synthesis participate in formaldehyde detoxification in the methylotrophic yeast Candida boidinii.Yeast,21, 341-350 (2004).

  13. H. Mukaiyama, M. Baba, M. Osumi, S. Aoyagi, N. Kato, Y. Ohsumi, and Y. Sakai.Modification of a ubiquitine-like protein Paz2 conducted micropexophagy through formation of a novel membrane structure.Mol. Biol. Cell,15, 58-70 (2004).[Cover Image]